Pathogen Detection Working Group - Technologies, Expertise, and Materials

Our individual NPRC laboratories have a wealth of knowledge and experience with various testing reagents, platforms, instrumentation, and protocols that we can share via consultation/troubleshooting and training. In addition, we have been able to identify, characterize, and archive a number of reagents, standards, and controls for pathogen detection. The quantities and availabilities vary. We encourage you to browse or search our inventory of available resources. Please contact the individual NPRCs for the availability of resources and consultation regarding ways we can support your research.

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Pathogen Technologies, Expertise, and Materials

California Emory Oregon Southwest Tulane Washington Wisconsin
Assays to measure the latent reservoir of SIV
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Blood sample processing
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Custom assay development
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DNA isolation (Gentra Kit)
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DNA isolation, cell-free virus
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Enzyme Immunoassay (EIA)
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Immunofluorescence Assay
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Immunohistochemistry
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In Situ Hybridization
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Isolation of NHP viruses in tissue culture
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Line Immunoassay
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Liquid Arrays: Multiplex Microbead Immunoassay (MMIA)- Luminex
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Manual and robotic nucleic acid isolation and purification
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Multiplex antibody detection
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Multiplex gene detection - DNA/RNA/protein - Nanostring
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Nucleic Acid Purification
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Plasma preparation from whole blood
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Polymerase Chain Reaction- Digital Droplet
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Polymerase Chain Reaction- Endpoint
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Polymerase Chain Reaction- Real Time
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Production of characterized virus stocks
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Production of custom SIV mutants
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Quantification of cell-associated SIV DNA by droplet digital PCR (ddPCR) or QPCR
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Quantitative viral outgrowth assay
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Rapid Dipstick or Flow Through Devices
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RNA isolation (Qiagen Rneasy Kit)
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Slide arrays
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Standard viral load assay
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Tissue culture
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Tissue processing (DNA AND RNA)
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VRNA extraction from plasma
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Western Immunoblot
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