Pathogen Detection Working Group - Technologies, Expertise, and Materials
Our individual NPRC laboratories have a wealth of knowledge and experience with various testing reagents, platforms, instrumentation, and protocols that we can share via consultation/troubleshooting and training. In addition, we have been able to identify, characterize, and archive a number of reagents, standards, and controls for pathogen detection. The quantities and availabilities vary. We encourage you to browse or search our inventory of available resources. Please contact the individual NPRCs for the availability of resources and consultation regarding ways we can support your research.
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Pathogen Technologies, Expertise, and Materials
Assays to measure the latent reservoir of SIV
Blood sample processing
Custom assay development
DNA isolation (Gentra Kit)
DNA isolation, cell-free virus
Enzyme Immunoassay (EIA)
Immunofluorescence Assay
Immunohistochemistry
In Situ Hybridization
Isolation of NHP viruses in tissue culture
Line Immunoassay
Liquid Arrays: Multiplex Microbead Immunoassay (MMIA)- Luminex
Manual and robotic nucleic acid isolation and purification
Multiplex antibody detection
Multiplex gene detection - DNA/RNA/protein - Nanostring
Nucleic Acid Purification
Plasma preparation from whole blood
Polymerase Chain Reaction- Digital Droplet
Polymerase Chain Reaction- Endpoint
Polymerase Chain Reaction- Real Time
Production of characterized virus stocks
Production of custom SIV mutants
Quantification of cell-associated SIV DNA by droplet digital PCR (ddPCR) or QPCR
Quantitative viral outgrowth assay
Rapid Dipstick or Flow Through Devices
RNA isolation (Qiagen Rneasy Kit)
Slide arrays
Standard viral load assay
Tissue culture
Tissue processing (DNA AND RNA)
VRNA extraction from plasma
Western Immunoblot